Detection Kit Will Speed up the Diagnosis Twice

Until the virus is defeated, all scientists around the world are still searching for a method to diagnose Covid-19 as quickly, cheaply, and accurately as possible. Such a method of SARS-COV-2 virus detection in Lithuania was proposed by the medical team of VU scientists, university reports. In the VU podcast “Mokslas be pamokslų” Professor Algirdas Utkus, Dean of the Faculty of Medicine, claimed that this method will allow Covid-19 to be detected twice as fast.

Diagnosis now takes 6 to 24 hours

According to the scientist, the gold standard for diagnosing Covid-19 worldwide today is the polymerase chain reaction (PCR) test, when a sample from the nasopharynx is taken to search for the virus RNA. This test is very relevant in the early stages of the disease, but when performed later, RNA may no longer be detected, as the infection descends down to the lower respiratory tract, where it can cause viral pneumonia.
A timely PCR test is, of course, accurate, but results can be obtained after 6 hours or even a day, as it requires preparatory work.

“First, a sample is taken from the nasopharynx or nasal mucosa, placed in a special medium and sent to the laboratory. The next step is to isolate the virus RNA from the sample, i.e., the genetic material which can be further examined by applying PCR to determine the presence of the virus RNA in the sample and to determine whether the person has the infection.”

Therefore, according to prof. Algirdas Utkus, the task of all scientists in the world is to improve the methods of diagnosing the disease, which would allow detecting this virus as quickly, accurately and cheaply as possible. The VU team of scientists is also keeping pace with foreign scientists and is trying to discover methods of virus testing that would facilitate the diagnosis of the virus.

“Our goal was to develop a nucleic acid detection kit for coronavirus and other viruses that would allow viruses to be detected directly from a sample without additional sample processing. This work has been successfully accomplished – a special detection kit has been developed to help identify the unique applications of certain specific genes,” says the geneticist.

An advanced and original direct virus detection method developed by VU researchers could halve the time it takes to detect the virus, which is very important when seeking to start treatment as early as possible and thus avoid possible serious complications of the disease.

The method is also suitable for detecting influenza
The principle of the newly discovered method is similar to the current testing as it also performs genetic tests that allow reliable identification of Covid-19 viral infection. “The difference and novelty are that samples for this test would be taken additionally from blood, urine and faeces. The new method we have developed for the detection of viral nucleic acids in these biological samples, which is already being applied under experimental conditions, will be put into practice after additional research. If everything works in the same way in working conditions, in March or April this method can be patented and used.

The scientist says that the new method detects the virus in a test tube by combining one or another biological sample with a liquid nucleic acid detection kit with unique targets – Orf 1a, RdRp and N genes. If these genes are detected, it can be confirmed that this biological sample is infected with SARS-COV-2 or another virus in cases of respiratory diseases.
According to the medical scientist, this method can also be used to identify other respiratory viruses that recur every year, such as influenza A, influenza B.

Although the new method of testing Covid-19 may not become the main one today, it would be useful and effective in addition to those already in use,” claims prof. Algirdas Utkus.